Journal: Stem Cell Research & Therapy

Article Title: Potential effect of amniotic fluid-derived stem cells on hyperoxia-induced pulmonary alveolar injury

doi: 10.1186/s13287-022-02821-3

Figure Lengend Snippet: Effect of stem cell treatment on biochemical markers. A Western blot analyses of IL-6 and RhoA in lung tissue in all groups. β actin was used for normalization. Images of the bands where obtained from the same gel with no cropping in between the bands. Full length blots are included in the Additional file Fig. S1. B , C Representative graphs for relative expression of IL-6 and RhoA respectively. D , E Representative graphs for quantification of MDA and TAC in lung tissue of all experimental groups. Data is represented in mean. n = 3 in all groups. a; significant compared to CG. b: significant compared to HG. c: significant compared to CFM-G. d: significant compared to SC-PG. e: significant compared to SC-TG. Pairwise comparison bet. Each 2 groups using Post Hoc Test (Tukey). Significance at p ≤ 0.05. Error bars represent S.E.M. Stem cell treatment significantly reduced IL-6, RhoA and increased TAC, while stem cell prophylaxis significantly reduced MDA. Abbreviations: CG; control group, HG; Hyperoxia group, CFM-G; Cell-free media-treated group, SC-PG; Stem cells-prophylactic group, SC-TG; Stem cells-treated group, Unt-G Untreated group, IL-6; interleukin 6, RhoA; Ras homolog family member A, MDA; malondialdehyde, TAC; total antioxidant capacity

Article Snippet: The lung tissues were homogenized in 1–2 ml of 5 mM cold potassium phosphate buffer (pH 7.4), centrifuged at 4000 rpm for 15 min at 4 °C and the supernatant was removed and stored at − 80 °C for assay of total antioxidant capacity (TAC) (Cat. No. TA 25 13, Biodiagnostics) and malondialdehyde (MDA) (Cat. No. MD 25 29, Biodiagnostics).

Techniques: Western Blot, Expressing, Comparison, Control

Journal: International Journal of Molecular Sciences

Article Title: Effect of Berberine on Glycation, Aldose Reductase Activity, and Oxidative Stress in the Lenses of Streptozotocin-Induced Diabetic Rats In Vivo—A Preliminary Study

doi: 10.3390/ijms21124278

Figure Lengend Snippet: Effect of berberine administration to the diabetic rats on the total antioxidant capacity (TAC) in the serum. The results are presented as an arithmetical mean ± SEM. The statistical significance of the results was evaluated by ANOVA followed by Tukey’s honestly significant difference (HSD) post-hoc test. The results were considered statistically significant if p < 0.05: * p < 0.05—statistically significant difference in comparison with the NDM group; ### p < 0.001—statistically significant difference between the DM + BRB and DM groups. NDM: non-diabetic control rats, DM: diabetic control rats, DM + BRB: diabetic rats treated with berberine at a dose of 50 mg/kg per os (p.o.) for four weeks.

Article Snippet: The serum total antioxidant capacity (TAC) was evaluated with a commercially available Cayman kit.

Techniques: IF-P, Comparison, Control

Journal: International Journal of Molecular Sciences

Article Title: Effect of Berberine on Glycation, Aldose Reductase Activity, and Oxidative Stress in the Lenses of Streptozotocin-Induced Diabetic Rats In Vivo—A Preliminary Study

doi: 10.3390/ijms21124278

Figure Lengend Snippet: Spearman’s r s correlation plot of biochemical parameters measured in the lenses and TAC in the serum; blue circles indicate positive correlations, red circles indicate negative correlations, both at p < 0.05; circles’ sizes correspond to correlation strength (the bigger the circle, the stronger the correlation). AGEs: advanced glycation end-products; AR: aldose reductase; SOD: superoxide dismutase; CAT: catalase; AOPP: advanced oxidation protein product; TBARS: thiobarbituric acid reactive substance; NPSH: non-protein sulfhydryl group; TAC: total antioxidant capacity.

Article Snippet: The serum total antioxidant capacity (TAC) was evaluated with a commercially available Cayman kit.

Techniques: